DOI: 10.22184/1993-7296.FRos.2020.14.6.542.549

The identification of the standard sizes of viral, aggregative and other particles using the virion flow analysis by the light-­scattering and fluorescence spectrum requires preliminary calibration of the measuring instrument. Calibration should be carried out according to specific geometric prototypes with specific dimensions. However, the size of viruses varies over a wide range of lengths ranging from nano- to submicrometer sizes. This leads to measurement uncertainty for a wide range of viruses. The software for widely implemented optical instruments is based on a simplified interpretation of the Mie scattering theory, extending it to almost the entire range of particle sizes, even submicrometer ones. The review examines the metrological problems of optical virometry and offers solutions to the issues of instrument calibration.


Разработка: студия Green Art